RNA-seq data of imbibed vs. mannitol- treated seeds in Arabidopsis thaliana Col - 0 and odr1 - 2 mutant

This study aims to investigate the transcriptomic differences between wild-type Arabidopsis thaliana Col-0 and the odr1-2 mutant in two experimental groups: imbibed seeds (under normal hydration conditions) and seeds subjected to 12-hour mannitol treatment (a common osmotic stress mimic).Using RNA sequencing to compare global gene expression profiles, we seek to identify differentially expressed genes (DEGs) associated with osmotic stress responses, seed imbibition, and the potential regulatory role of the ODR1 gene. Our analysis will clarify how the ODR1 mutation alters transcriptional dynamics under both normal and osmotic stress conditions, thereby providing insights into the molecular mechanisms underlying stress tolerance and seed physiology in Arabidopsis. Overall design: Transcriptome sequencing was conducted on 12 samples (with three biological replicates per group), derived from Arabidopsis thaliana Col-0 (wild type) and odr1-2 (mutant). All samples were prepared from after-ripened seeds stratified at 4°C for 2 days (to induce dormancy break), followed by two treatments: 12 h imbibition in water or 12 h treatment with 300mM mannitol.