EFFECT OF A MIXTURE OF BACTERIA STRAINS ON STRAWBERRY

Background: Research conducted in field experiments 2014-2017 years the site of production of wild strawberry mother plants in the agricultural cooperative \"Gardens of Siberia\" in the Novosibirsk region (Russia) in the subzone of the drained forest-steppe of the Ob region. The soil is grey forest. The study aims to evaluate the multifunctional effect of a preparative mixture of strains of saprotrophic bacteria of the genus Bacillus on garden strawberry plants under conditions of production of wild strawberry mother plants. Results: The authors found that the pre-planting treatment of strawberry seedlings with a suspension of a mixture of strains of saprotrophic bacteria of the genus Bacillus (B. amyloliquefaciens (RCIM) B-10642, B. amyloliquefaciens (RCIM) B-10643, B. subtilis (RCIM) B-10641 - in equal proportions, at a concentration of 1 × 105 CFU/ml) increased their survival by an average of 20.5% relative to the control, providing a pronounced anti-stress effect. The degree of damage to the leaves of strawberry white spot decreased 1.2-1.8 times due to the drug's immunizing effect. Treatment with a mixture of bacterial strains stimulated an increase in the length of the aerial part by 15%, the length of the root system - by 19%, biomass of strawberry plants - by 20%, and also stimulated vegetative reproduction of strawberries - the number of daughter outlets increased by 31.8%. Conclusions: The preparative mixture of bacterial strains has a multifunctional effect on plants. It is a promising tool for expanding the arsenal of biological methods for increasing the efficiency and environmental stabilization of the production of strawberry planting material.背景：本研究于2014—2017年，在俄罗斯新西伯利亚州鄂毕河区域疏干森林草原亚带的"西伯利亚花园"（Gardens of Siberia）农业合作社野生草莓母本繁育基地开展田间试验，供试土壤为灰色森林土。本研究旨在评估芽孢杆菌属（Bacillus）腐生细菌菌株混合制剂，在野生草莓母本繁育条件下对草莓植株的多功能调控效应。结果：研究发现，采用等比例混合的芽孢杆菌属腐生细菌菌株悬液（菌株为解淀粉芽孢杆菌（B. amyloliquefaciens）RCIM B-10642、解淀粉芽孢杆菌（B. amyloliquefaciens）RCIM B-10643、枯草芽孢杆菌（B. subtilis）RCIM B-10641，浓度为1×10^5菌落形成单位/毫升（CFU/ml））对草莓幼苗进行定植前处理，较对照组平均提升20.5%的幼苗存活率，展现出显著的抗胁迫效应。依托该制剂的免疫诱抗作用，草莓白斑病的叶片受害程度降低1.2~1.8倍。该细菌菌株混合制剂处理可促进草莓地上部株高增长15%、根系长度增加19%、植株生物量提升20%，同时刺激草莓营养繁殖，子株匍匐茎苗数量增加31.8%。结论：该细菌菌株混合制剂对植物具有多功能调控效应，是拓展生物防治手段库、提升草莓种苗繁育效率与环境稳定性的潜在有效工具。