Effect of lncMGC on gene expression of kidney mesangial cells under SD or TGF-ß treatment [ATAC-seq]

To investigate the function of long noncoding RNA lncMGC in WT and lncMGC KO mouse kidney mesangial cells treated with TGF-ß. Assay for Transposase-Accessible Chromatin (ATAC) sequencing on 4 types of kidney messangial cell samples: isolated from wild type (WT) or lncMGC knockout (lncMGC_KO) mouses, each treated with either SD or TGF-ß, namely WT_SD, WT_TGF, KO_SD and KO_TGF. Overall design: Study the genomic regions with open chromatin based on the data from ATAC-seq to reveal the functions of lncMGC on chromatin structures in kidney mesangial cells.