O-glycan loss can affect HNV G conformation.

A & B) Normalized mAb binding to wt or mutant HeV G (A) or NiV G (B). All antibody binding levels are normalized to the CSE of each mutant (as measured by HA levels). N = 3. C & D) Conformational changes of wt or mutant HeV G (C) or NiV G (D) as measured by Mab45 (white) and Mab26 (black) upon the addition of 100nM ephrinB2. All values are normalized to antibody binding levels when no (0nM) ephrinB2 was added. Averages and standard deviations are shown. N = 3. Statistically significant differences, as determined by a Student’s t-test, are marked with an asterisk (p<0.05).