Differential gene expression CDH1,BUB1B,MMP9,and FOXM1 affect the occurrence and development of endometrial adenocarcinoma:a study based on Differential gene expression and immunohistochemistry analysis

Endometrial carcinoma (EC) is a major malignant tumor of the female reproductive system, its pathogene-sis is complex, not yet fully described. Over the past decade, RNA sequencing (RNA-seq) has become an indispensable tool for transcriptome-wide analysis of differential gene expression and differential splicing of mRNAs. In order to carry out molecular typing and risk analysis of tumors and guide precise treatment,we used RNA-seq to detect genes expressed in samples of endometrioid adenocarcinoma and normal endometrial tissues while 3329 up regulated and 1328 down regulated differential gene expression(DEGs) were detected. Gene Ontology (GO) and pathway enrichment analysis of DEGs were carried out. The DEGs in EC samples mainly affect the regulation of gene expression, cell cycle and apoptosis processes via related genes. The main signaling pathway involved in cellular molecular processes of EC is the PI3K-Akt pathways. Twenty core genes were screened on the basis of the PPI network,among which CDH1, BUB1B, MMP9 and FOXM1 were preliminarily verified by immunohistochemistry.CDH1, BUB1B, MMP9, and FOXM1 are important markers for many other human diseases, such as rhabdomyosarcoma and breast cancer. Our finding aids in molecular typing, risk analysis of tumors, and can inspire research on the molecular mechanism of tumor occurrence.