CRISPR mediated deletion of RP11-326A19.4/CARMAL. CRISPR mediated deletion of RP11-326A19.4/CARMAL

Deletion experiment aimed at understanding the role of lncRNA RP11-326A19.4 /CARMAL via its deletion. The impact on of the deletion on the transcriptome was assessed by array analysis. Overall design: HEK293T cells were transfected with dCas9-VPR alongside either 2 gRNA expression constructs spanning exon 1 of RP11-326A19.4/CARMAL or a tracrRNA only for 48 h. Single clones were then obtained by cell sorting and genotyped. RNAs from a deleted clone and 3 control clones, obtained over 4 passages, were isolated and analyzed on human HuGene-2_0-ST (Affymetrix) arrays. Each Wtx/KOx pair represents a matched set from passage number x.