Data for: The indoor mycobiomes of daycare centers are affected by occupancy and climate

With an alarming increase in chronic diseases like childhood asthma and allergies, there is an increased focus on the exposure of young children to indoor biological and chemical air pollutants. Our study of 125 daycares throughout Norway demonstrates that the indoor mycobiome not only reflects cooccurring outdoor fungi but also includes a high abundance of yeast and mold fungi with an affinity for indoor environments., Sampling
A list of Norwegian daycare centers was retrieved from the Norwegian Directorate of Health (Helsedirektoratet). The list was sorted alphabetically after counties and municipalities, and the first five municipalities in each county were selected for the study. Likewise, the first 3–4 daycare centers in each of these municipalities were chosen as candidate sites for dust sampling. Sampling kits containing five FLOQSwabs (Copan Italia spa, Brescia, Italy) and a questionnaire were sent to the selected daycare centers with specifications to perform dust sampling on doorframes: (1) outdoor, (2) main room and (3) bathroom. If the daycare had two different departments, we requested to repeat the sampling in (4) the main room and (5) the bathroom of the second department as well. Overall, 572 samples were retrieved from a total of 125 studied daycare centers, and upon arrival the swabs were stored at -80 ºC until DNA extraction.
DNA extraction and metabarcoding
Samples were prepared and...,