Data for: The indoor mycobiomes of daycare centers are affected by occupancy and climate

With an alarming increase in chronic diseases like childhood asthma and allergies, there is an increased focus on the exposure of young children to indoor biological and chemical air pollutants. Our study of 125 daycares throughout Norway demonstrates that the indoor mycobiome not only reflects cooccurring outdoor fungi but also includes a high abundance of yeast and mold fungi with an affinity for indoor environments., Sampling A list of Norwegian daycare centers was retrieved from the Norwegian Directorate of Health (Helsedirektoratet). The list was sorted alphabetically after counties and municipalities, and the first five municipalities in each county were selected for the study. Likewise, the first 3–4 daycare centers in each of these municipalities were chosen as candidate sites for dust sampling. Sampling kits containing five FLOQSwabs (Copan Italia spa, Brescia, Italy) and a questionnaire were sent to the selected daycare centers with specifications to perform dust sampling on doorframes: (1) outdoor, (2) main room and (3) bathroom. If the daycare had two different departments, we requested to repeat the sampling in (4) the main room and (5) the bathroom of the second department as well. Overall, 572 samples were retrieved from a total of 125 studied daycare centers, and upon arrival the swabs were stored at -80 ºC until DNA extraction. DNA extraction and metabarcoding Samples were prepared and...,随着儿童哮喘、过敏等慢性病的患病率呈令人警觉的上升趋势，学界对幼儿暴露于室内生物与化学空气污染物的关注度日益提升。我们对挪威全境125家日托中心开展的研究表明，室内真菌组（mycobiome）不仅能够反映同期共存的室外真菌群落特征，还包含大量偏好室内环境的酵母菌与霉菌。 采样 研究从挪威卫生署（Norwegian Directorate of Health, Helsedirektoratet）获取了挪威日托中心名录。该名录按郡与自治市的字母顺序排序，我们选取每个郡内排名前五的自治市纳入研究。同样，我们选取这些自治市内排名前3至4家的日托中心作为粉尘采样的候选场地。 研究人员向入选的日托中心寄送了包含5支FLOQSwabs采样拭子（Copan Italia spa，意大利布雷西亚）与一份调查问卷的采样套装，并明确要求在三处门框处开展粉尘采样：(1) 室外门框，(2) 主活动室门框，(3) 浴室门框。若该日托中心设有两个不同的分部，则需额外对(4) 第二分部的主活动室门框与(5) 第二分部的浴室门框进行重复采样。 最终，我们从纳入研究的125家日托中心共获取572份样本，拭子在运回后于-80℃低温保存，直至开展DNA提取。 DNA提取与元条形码测序（metabarcoding） 样本制备及后续实验步骤详见原文后续内容……