Serum proteomic profiles in keratoconus, post-laser vision correction ectasia, and pellucid marginal degeneration

This dataset accompanies the manuscript titled 'Common and distinct features in serum proteomic profiles in keratoconus, post-laser vision correction ectasia, and pellucid marginal degeneration’ by Jaskiewicz-Rajewicz et al., submitted to Investigative Ophthalmology & Visual Science (IOVS). In this study serum samples from 93 patients with keratoconus (KTCN), 10 patients with post-laser vision correction (PLVC) ectasia, 4 patients with pellucid marginal degeneration (PMD), and 44 controls were profiled using tandem matrix-assisted laser desorption/ionization time of flight/time of flight mass spectrometry (MALDI-TOF/TOF MS/MS). Clinical and environmental variables (e.g. allergy/atopy/asthma, eye rubbing intensity), together with ophthalmologic parameters (K1, K2, Kmax, TCT, anterior/posterior elevation), were assessed in the principal component analysis (PCA), Weighted Gene Co-expression Network Analysis (WGCNA), Mann–Whitney feature testing, linear modelling, and Spearman correlation and other analyses. The data was processed on 10 December 2025. Mendeley Supplementary Table 1. Table containing sample metadata, including Sample ID (short), corresponding Patient ID, study subgroup, sex, and age. Mendeley Supplementary Table 2. Table containing unprocessed m/z peak intensity values detected in analyzed spots, indexed by the corresponding Sample ID (short).本数据集配套于Jaskiewicz-Rajewicz等人提交至《眼科学与视觉科学研究（Investigative Ophthalmology & Visual Science，IOVS）》的题为《圆锥角膜、激光术后角膜扩张与透明边缘变性患者血清蛋白质组谱的共性与差异特征》的研究手稿。 本研究采用串联基质辅助激光解吸电离飞行时间/飞行时间质谱（tandem matrix-assisted laser desorption/ionization time of flight/time of flight mass spectrometry，MALDI-TOF/TOF MS/MS），对93例圆锥角膜（keratoconus）患者、10例激光术后角膜扩张（post-laser vision correction ectasia）患者、4例透明边缘变性（pellucid marginal degeneration）患者及44例健康对照的血清样本进行了蛋白质组谱分析。研究中评估了临床与环境变量（如过敏、特应性反应、哮喘、揉眼强度）及眼科参数（K1、K2、Kmax、TCT、前/后表面高度），并将其应用于主成分分析（principal component analysis，PCA）、加权基因共表达网络分析（Weighted Gene Co-expression Network Analysis，WGCNA）、曼-惠特尼特征检验、线性建模、斯皮尔曼相关性分析等多种分析方法。 本数据集于2025年12月10日完成数据处理。 Mendeley补充表1：包含样本元数据的表格，涵盖短格式样本ID、对应患者ID、研究亚组、性别及年龄信息。 Mendeley补充表2：包含分析斑点中检测到的未处理m/z峰强度值的表格，以短格式样本ID作为索引。