Dataset of identification of secondary metabolites and Antibacterial Activity of Lantana camara L. leaf extract against Staphylococus aureus and Escherichia coli

The folder named 1-Database-Lantana camara leaf screening-2019 contains four files. The 1-Dataset-2019 file contains a table 1 which shows the class of alkaloids, tannins, saponins, phenolic compounds and quinone identified in the L. camara leaf extract according to the extract drying method. The 2-Dataset-2019 and the 3-Dataset-2019 files contain table 2 and table 3, which show the yield of dry extract which was dried in an oven and the yield of extract dried in a rotary evaporator, respectively. Besides, the 4-Dataset-2019 includes the concentration of extract of Lantana camara leaves and its diameter of inhibition halo against S. aureus and E.coli and its positive control. This plant belongs to the verbenaceae family and is used for several treatments in folk medicine.The plant extract was prepared using the maceration method, where 200 g of the powder of the leaves of L. camara was mixed with 2 L of ethanol at the concentration of 90%, in the proportion of 1g/10mL, and occasionally stirred for 7 days. The mixture was stored in a dry environment protected from direct light and the daily temperature was an average of 26ºC. After filtration, the yield of the dry extract was determined. And, one part of the liquid extract was dried in an oven at 80°C for 8 hours, and the other part was placed in a rotary evaporator at reduced pressure and at a temperature of 42 °C for 6 hours. After being dried, the extract was analyzed in search for bioactive metabolites. In addition, the evaluation of the antibacterial activity of the ethanolic extract from the leaves of L. camara was carried out using the Kirby-Bauer disk diffusion method. The E. coli strains were obtained from a stool sample cultured on TCBS agar (thiosulfate, citrate, bile and sucrose). While the S. aureus strains were obtained from a pus wound sample cultured on blood agar, they were used.名为"1-Database-Lantana camara leaf screening-2019"的文件夹共包含4个文件。其中1-Dataset-2019文件内含表1，展示了依据提取物干燥方式，从马缨丹（Lantana camara）叶片提取物中鉴定出的生物碱、单宁、皂苷、酚类化合物及醌类化合物的类别。2-Dataset-2019与3-Dataset-2019文件分别包含表2与表3，前者展示了烘箱干燥提取物的干膏得率，后者则展示了旋转蒸发仪干燥提取物的得率。此外，4-Dataset-2019文件包含马缨丹叶片提取物的浓度数据，以及其针对金黄色葡萄球菌（Staphylococcus aureus，简称S. aureus）和大肠杆菌（Escherichia coli，简称E. coli）的抑菌圈直径，同时附带对应阳性对照的相关数据。 该植物隶属于马鞭草科（Verbenaceae），在民间医学中被用于多种病症的治疗。本研究采用浸渍法制备植物提取物：称取200g马缨丹叶片粉末，与2L浓度为90%的乙醇混合，料液比为1g/10mL，期间间断搅拌，总浸渍时长为7天。将混合体系置于避光干燥环境中储存，日均温度维持在26℃左右。过滤完成后，测定干提取物的得率。随后将部分液态提取物置于80℃烘箱中干燥8小时，剩余部分则置于减压条件下、42℃的旋转蒸发仪中干燥6小时。干燥完成后，对提取物展开生物活性代谢物的检测分析。 此外，本研究采用柯比-鲍尔纸片扩散法（Kirby-Bauer disk diffusion method），评估马缨丹叶片乙醇提取物的抗菌活性。大肠杆菌菌株分离自经硫代硫酸盐-柠檬酸盐-胆盐-蔗糖（TCBS）琼脂培养的粪便样本；而金黄色葡萄球菌菌株则分离自经血琼脂培养的创面脓液样本，并将其用于本次抗菌活性评估实验。