mRNAseq of primary hepatocytes treated in vitro with Itaconate, Lipid, or Itaconate + Lipid

primary hepatocytes isolated from wildtype mouse livers and treated overnight in complete media with 10mM sodium itaconate in the presence or absence of a 1:10 dilution of sterile, chemically defined lipid mixture 1 (Sigma-Aldrich) Overall design: Gene expression analysis from four replicates of primary hepatocytes were untreated (control), treated with sodium itaconate alone, lipid mix alone, or itaconate and lipid mix overnight