Patient-derived organoid fibroblast co-cultures closely mimic tumor cell heterogeneity of colorectal cancer

Here we compare RNA expression profiles of colorectal cancer organoids grown in four different conditions, namely standard organoids medium, minimal medium without growth factors or with normal or cancer associated fibroblasts. We found majorly different expression profiles between conditions and more heterogeneous tumor cell population in organoids grown with fibroblasts. Overall design: Evaluation of RNA expression changed caused by co-culturing of tumor cells and fibroblasts