Transport assay of the hSLCO5A1 protein with Tritium-labelled substrates in X. laevis oocytes.

X. laevis oocytes (8–12 oocytes) were injected with the cRNA of the WT SLCO5A1 or its L33F mutant, or with the control (Tris-HCl). Oocytes were incubated with 1 µCi/ml Tritium-labelled substrate and 0.04 µCi/ml [14C]sucrose at room temperature for 30 minutes. [14C]sucrose served as internal leakage control. Radioactivity was measured using a Beckman scintillation counter. Mean CPM (counts per minute) values with standard deviation are displayed.