Long-term recovery from freezing in Belgica antarctica

This study examines how energy reserves and gene expression change between Belgica antarctica larvae that did and did not go through a freezing and recovery event (frozen and control). The midges were exposed to -5C for 24h and recovered for 15 days. Samples were taken immediately after thaw, 1, 3, 7, and 15 days of recovery for biochemical assays and RNA-sequencing, Dataset \"survival\" shows survival rate between frozen and control Datasets \"carb results\", \"lipid results\", and \"protein results\" show changes in energy reserves between frozen and control Dataset \"KEGG results\" show pathways elicited by differential gene expression between frozen and control本研究旨在探究经历冷冻-复苏处理（冷冻组）与未接受该处理的对照组南极无翅摇蚊(Belgica antarctica)幼虫的能量储备与基因表达变化。受试个体于-5℃环境下暴露24小时，随后复苏培养15天。研究人员分别于解冻后即刻、复苏第1、3、7及15天采集样本，用于生化指标检测与RNA测序(RNA-sequencing)。 数据集"survival"展示了冷冻组与对照组的存活率数据； 数据集"carb results"、"lipid results"与"protein results"呈现了两组间的能量储备变化情况； 数据集"KEGG results"则揭示了冷冻组与对照组间差异基因表达所富集的通路信息。