Effect of graded normobaric hypoxia on Genome-wide expression in healthy individuals. Effect of graded normobaric hypoxia on Genome-wide expression in healthy individuals

The regulation of oxygen homeostasis is critical in physiology and disease pathogenesis. Studying transcriptomic profiles after hypoxia exposure could potentially uncover valuable biomarkers for predicting responses. To this end, 6 healthy individuals were recruited and exposed to graded normobaric hypoxia (FiO2 ~ 0.19 up till 0.13 in steps of 2% decrement in 10 minutes). A blood sample was collected before (at room air) and after the graded normobaric hypoxia exposure (at FiO2 ~ 0.13) for the gene-expression profiles. Results provide important information about early molecular differences induced by our experimental protocol in young, healthy individuals. Overall design: Peripheral Blood samples were obtained from healthy individuals. RNA was extracted. Labeled cRNA was hybridized to Illumina Human HT-12 V4 Beadchips.