Genome-Wide Identification and Characterization of Main Histone Modifications in Sorghum Decipher Regulatory Mechanisms Involved by mRNA and Long Noncoding RNA Genes

Post-translational modifications
of histones play an important
chromatic role of a transcript activity in eukaryotes. Even though
mRNA and long noncoding RNA (lncRNA) genes share similar biogenetic
processes, these transcript classes may differ in many ways. However,
knowledge about the crosstalk between histone methylations and the
two types of sorghum genes is still ambiguous. In the present study,
we reveal the genome-wide distribution of six histone modifications,
namely, di- and trimethylation of H3K4 (H3K4me2 and H3K4me3), H3K27
(H3K27me2 and H3K27me3), and H3K36 (H3K36me2 and H3K36me3) in sorghum
and analyze their functional relationships. Unlike other histone methylation,
the codecoration of H3K4me3 and H3K36me3 is negatively associated
with the production of lincRNAs in the context of active expression
of mRNA genes. Our data demonstrated that H3K4me3 may act as a complementary
component to H3K36me3 in the transcriptional regulatory process. Moreover,
we observe that both H3K4me3 and H3K36me3 are involved in the negative-going
regulation of plant lincRNA and mRNA genes. Our data provide a genome-wide
landscape of histone methylation in sorghum, decrypt its reciprocity,
and shed light on its transcriptional regulation roles in mRNA and
lncRNA genes.