Apparent equilibrium dissociation constants (K<sub>D</sub>) for the Sso7d mutants.
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For each mutant, data from at least two independent experiments were fit globally to a four parameter logistic model using non-linear least squares regression for estimation of KD. The corresponding 68% confidence intervals are shown in parentheses. aNot determined. The binding isotherm at pH 4.5 did not reach saturation even with 20–30 µM concentration of Sso7d-his-hFc and Sso7d-ev-hFc; consequently, an apparent KD for the binding interaction at pH 4.5 could not be calculated.
创建时间：
2012-11-07
