poly A+ RNA sequencing of cell cycle-synchronized RNA from U2OS cells. poly A+ RNA sequencing of cell cycle-synchronized RNA from U2OS cells

We performed paired-end poly A+ RNA sequencing using total RNA isolated from U2OS cells that are synchronised into G1, G1/S, S, G2 and M phase of cell cycle. Overall design: M-phase cells were collected using Nocadozole treatment. G1 cell are isolated using Nocadozole arrest followed by release. G1/S, S and G2 cells were collected by performing double-thymidine block and release.