Co-immunization with two recombinant Newcastle disease viruses expressing ILTV glycoprotein B and H9N2 AIV HA confers cross-protection against three avian pathogens

File 1: Viral growth kinetics in BHK-21 and CEF cells. The cells were coinfected or individually infected with rTS-H9/rTS-gB at an MOI of 0.01. Viral titers in culture supernatants collected at 12-hour intervals were determined by IFA on the indicated cell lines.
File 2: The original image of Western blot analysis.BHK-21 or CEF cells were coinfected or individually infected with rTS-H9/rTS-gB at an MOI of 0.1. Western blot analysis of BHK-21 (F) and CEF (G) cell lysates harvested at 48 hpi. Proteins were detected using specific antibodies against HA (H9N2 AIV), gB (ILTV), and NDV nucleoprotein (NP). The mouse anti-actin polyclonal antibody served as a loading control.
File 3: Protective efficacy in SPF chicks. Antibody titers against NDV, AIV and ILTV after immunization, and the date of survival rate, clinical symptoms, tissue viral load and virus shedding after challenging with NDV, AIV and ILTV in SPF chicks
File 4: Protective efficacy in commercial chicks. Antibody titers against NDV, AIV and ILTV after immunization, and the date of survival rate, clinical symptoms, tissue viral load and virus shedding after challenging with NDV, AIV and ILTV in commercial chicks