Bulk RNA-seq of Quiescent and Nutlin3a-Induced Senescent mouse pulmonary fibroblasts. Bulk RNA-seq of Quiescent and Nutlin3a-Induced Senescent mouse pulmonary fibroblasts

Purpose: Use RNA-seq strategy to characterize the differentially expressed genes and significant transposable elements in quiescent and nutlin3a-induced senescent pulmonary fibroblasts to identify the factors and possible neoantigens in activating the cytotoxic T cell immunity. Methods: FACSorted primary pulmonary fibroblasts were utilized for the preparation of bulk RNA-seq libraries. Quiescence was induced by starvation and senescence was induced by nutlin 3a treatment. Overall design: Examination of differentially expressed genes and significant transposable elements within quiescent and nutlin3a-induced senescent pulmonary fibroblasts