DNA methylation analysis of colorectal cancer SW48/YB5 cell line treated with the HH1 compound. DNA methylation analysis of colorectal cancer SW48/YB5 cell line treated with the HH1 compound

YB5 is a colon cancer cell line derived from the SW48 colon cancer cell line and contains a DNA methylated and silenced CMV promoter driving expression of a GFP reporter. The cells were treated with 10 micromolar concentration of the HH1 compound or with DMSO as a control. Reduced representation bisulfite sequencing (RRBS) was performed after the treatment to analyze DNA methylation at CpG sites. Overall design: Biological triplicates were performed for HH1 and DMSO control. Untreated cells were analyzed in a biological duplicate.