Aqueous Artemisia argyi extract mitigates acute lung injury in association with coordinated alterations in gut microbiota, metabolic homeostasis, and pulmonary inflammatory gene expression

his study investigated the metabolic alterations in colonic contents associated with aqueous extract of Artemisia argyi (AEAA) intervention in a lipopolysaccharide (LPS)-induced acute lung injury (ALI) mouse model. Male C57BL/6 mice were randomly divided into control, LPS, and AEAA treatment groups with low-, medium-, and high-dose administration.
AEAA was administered by oral gavage for 28 consecutive days. On day 29, ALI was induced by intratracheal instillation of LPS (5 mg/kg) in the LPS and treatment groups, while control mice received sterile saline. Colonic content samples were collected 8 hours after LPS administration.
Untargeted metabolomics analysis was performed using liquid chromatography¨Cmass spectrometry (LC-MS). Quality control (QC) samples were included to ensure analytical stability. The metabolomic profiles of colonic contents were analyzed to explore gut metabolic alterations and potential mechanisms underlying the protective effects of AEAA in acute lung injury.
This dataset provides raw LC-MS data of colonic content metabolomics and can be used to investigate gut-related metabolic pathways involved in inflammation and natural product intervention.