Cystic fibrosis transmembrane conductance regulator (CFTR) is an ATP-regulated, cAMP-activated chloride channel located in the apical membrane of many epithelial secretory cells. Here we report clonin

PubMed Central1996-05-28 更新10

Supplementary data: Simple and efficient custom TALE genes synthesis via twin primer assembly

TALE效应子

分子克隆

Supplemental Material. Some sequencing results of correct assembly pGEM-USER pentamer vectors. RVD residues are highlighted in red.

Taylor & Francis Group2024-05-15 更新20

Efficiency of cloning of pX-GFP at different restriction-ligation times.

分子克隆

限制性内切酶活性

Restriction/ligations were performed from 5 to 30 minutes, and either immediately transformed in E.coli (Digest an heat inactivation/no) or incubated 5 minutes at 50°C and 5 minutes at 80°C (Digest an

NIAID Data Ecosystem20

<p>Strains, plasmids and oligonucleotides used in this study.</p>

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