Genome-wide analysis of osteosarcoma for comparison to healthy bone tissue

Analysis of common pediatric osteosarcoma cell lines SaOS2, U2OS and 143B and two patient derived xenograft samples in comparison of osteoblast cell line hFOB1.19. Pediatric osteosarcomas (OS) exhibit extensive genomic instability that has complicated the identification of new targeted therapies. Here we report that the vast majority of 108 patient tumor samples and patient-derived xenografts (PDXs), which display an unusually dilated endoplasmic reticulum (ER) haver reduced expression of four COPII vesicle components that trigger aberrant accumulation of procollagen-I protein within the ER. OS cell lines were used as an experimentally tractable system. Total RNA obtained from cells and tumor tissue of PDXs