Expression data from limbic area of mouse brain

Microarray results revealed that EEA treatment induced variations in several genes associated with pro-inflammatory and dopaminergic, cholinergic, glutamatergic, and serotonergic synapses. DNA microarray analysis was conducted on isolated RNAs extracted from the limbic area of EEA-treated brains Briefly, purified aRNA was fragmented using the GeneAtlas 3´ IVT Express Kit and hybridized for 16 h at 45°C using the GeneChip MG-430 PM microarray (Affymetrix Inc., Santa Clara, CA, USA). The chip was washed and stained in the Gene Atlas Fluidics Station 400 (Affymetrix Inc., Santa Clara, CA, USA), and the resulting image was scanned using the GeneAtlas Imaging Station (Affymetrix Inc., Santa Clara, CA, USA). Data analysis was performed using the expression console and Visualization and Integrated Discovery (DAVID) v6.8 (National Institute of Allergy and Infectious Diseases (NIAID). Compared with the control (vehicle-treated group), fold-changes in the expression of the imipramine- or EEA-treated groups were calculated and converted to linear data.