Expression data of human primary placenta trophoblast cells, at different time points following seeding

Cellular senescence is a permanent state of cell cycle arrest that protects the organism from tumorigenesis and regulates tissue integrity upon damage. Recently, several studies have shown that senescence plays a role in embryonic and placental development. Molecular markers of senescence are expressed in human syncytiotrophoblast, but the molecular mechanisms that govern senescence in these cells are not yet understood. To unravel the molecular mechanisms that mediate the impact of senescence on the placenta, we studied human syncytiotrophoblast in culture. We isolated cytotrophoblast cells from human term placentas. In culture, cytotrophoblast cells spontaneously differentiate into syncytium, creating the large multinucleated syncytiotrophoblast structure that can be monitored and studied for its molecular and cellular characteristics (Kliman et al., 1986; Li and Schust, 2015). We performed mRNA profiling on two human term placenta trophoblast cultures, at 1.5, 2, 3 and 5 days after seeding. To gain an insight into the molecular mechanisms activated during fusion of the placental syncytiotrophoblast, we performed mRNA profiling, using affymetrix PrimeView, on primary trophoblasts obtained independently from two human term placentas, at 1.5, 2, 3 and 5 days after seeding.