soil fungal metagenome

Soil DNA was extracted for subsequent amplicon sequencing of the bacterial 16S and fungal ITS rRNA genes. At each plot, nine cores were collected on a S-shape transect at 0-10 cm depth using a 2.5-cm corer and composited to create one soil sample, resulting in a total of 35 soil samples across sites. Soils were placed in polyethylene bags, stored on ice, and transported to the laboratory within one to two days.