Temporal study of the effect of acute systemic inflammation on the cerebral endothelial cells (CECs) and microglia in mice

To capture the global gene expression patterns in the CECs and microglia following acute systemic inflammation, we injected the mice with LPS and sacrificed them after 30 min, 1 hr and 2 hrs, immediately followed by the isolation of the brains and dissociation to yield single cell suspension, immunolabelling and finally flow assisted cell sorting of CECs (CD45- CD13- CD31+) and microglia (CD45 low-mid CD11b+). RNA was then isolated from the cells followed by RNA-seq. mRNA profiles of CECs and microglia isolated from the brains of mice (C57BL6, Wild type, 2-3 months old) injected (intra peritoneal) with 10mg/kg LPS for 30 min, 1 hr and 2 hrs or PBS n= 3 (males) each group.