The nuclear granule Paraspeckles interacts with the chromatin remodeling SWI/SNF complex to regulate alternative splicing

There are three major goals of the study 1) Understand if the differential effect of depletion of ARID1 paralogs on the trancriptome of HEK293FRT cells 2) Understand if NEAT1 depletion effects any alternative splicing pathways in HEK293FRT cells 3) Is the regulation of alternative splicing pathways by NEAT1 ARID1B dependent? Overall design: siRNA specific to either ARID1A, ARID1B or NEAT1 was used for experimental data sets. For both the controls, Wildtype or Parental a scrambled siRNA was used. In brief, 2 rounds of transfection with siRNA was employed with 24 hour interval in between each transfection. All the cells were harvested post 72 hours of first siRNA transfection and processed for RNA extraction.