The embryonic DPPA3 gene stimulates the expression of pregnancy-related genes in bovine endometrial cells

This dataset contains supplementary materials associated with the study investigating extracellular vesicle (EV) mRNA transfer from bovine embryos to endometrial cells. The dataset includes (1) a table of primers used for quantitative PCR (qPCR), specifying primer sequences and target genes; (2) an image demonstrating the uptake of GFP mRNA by bovine endometrial epithelial cells following EV supplementation, confirming successful mRNA translation; and (3) a table of differentially expressed genes from RNA sequencing analysis, detailing gene names, log fold changes (logFC), p-values, and functional annotations. This dataset provides valuable resources for researchers studying EV-mediated communication, embryo-maternal interactions, and gene expression changes in response to EV-derived mRNA. No ethical or legal restrictions apply to the dataset, and it can be freely used for comparative analyses, validation studies, or further exploration of transcriptomic changes in reproductive biology...., , # The embryonic DPPA3 gene stimulates the expression of pregnancy-related genes in bovine endometrial cells

Dataset DOI: [10.5061/dryad.tht76hf98](10.5061/dryad.tht76hf98)

## Description of the data and file structure

This dataset was generated as part of a study investigating the role of extracellular vesicle (EV)-derived mRNA in embryo-maternal communication in cattle. The experimental efforts focused on identifying full-length mRNA transcripts within EVs secreted by bovine embryos and assessing their impact on gene expression in maternal endometrial epithelial cells.

To achieve this, in vitro fertilized bovine embryos were cultured, and EVs were isolated from the culture media. RNA sequencing was performed to identify mRNA transcripts within these EVs. A key transcript, DPPA3, was selected for further functional analysis. Endometrial epithelial cells were supplemented with either embryo-derived EVs or in vitro transcribed DPPA3 mRNA to evaluate gene expression changes via RNA seq...,