mRNA and lncRNA microarray of vCAF modulating intrahepatic cholangiocarcinoma progression

We found that the isolated and expanded MCAM positive vCAF subpopulations significantly accelerated ICC growth. To further investigate the effect of vCAF on ICC cells (GFP-FRH or GFP-RBE), we co-cultured the vCAF with GFP ICC cells or ICC cells alone for seven days, then we sorted the GFP-FRH or GFP-RBE cells via FACS and conducted transcriptome microarray analysis of GFP ICC cells alone or vCAF co-cultured GFP-ICC cells. microarray experiments, GFP-FRH or GFP-RBE cells alone or co-cultured with vCAFs for 7 days.Then GFP-FRH or GFP-RBE were sorted by FACS and Total RNA was extracted with Trizol and hybridized to custom lncRNA and mRNA microarray