Epigenome analysis of hESCs undergoing neuronal differentiation

Genome wide DNA methylation profiling of human embryonic stem cells of hESCs undergoing neuronal differentiation at four distinct time-points. hESCs are differentiated towards neuronal cells for 13 days, and sampled for downstream analysis at day 0, 6, 10 and 13 . Cells exposed to 50, 100, 200 and 400 nM citalopram from Day 1 were charachterised at Day 6, 10 and 13. The Illumina Infinium MethylationEPIC_v-1-0_B3 was used to obtain DNA methylation profiles accross approximately 850k CpGs in differentiating cells. Bisulphite converted DNA from the 89 samples were hybridised to the Illumina Infinium MethylationEPIC BeadChip Submitters state "raw data IDAT files are not provided due to data sensitivity. We have used embryonic stem cells and there is a possibility of tracing back data to the donor, thus we are not allowed to share raw data".