Effects of maternal Type 1 Diabetes on neonatal microbiomes

This is the Supporting Dataset for the manuscript 'Effects of maternal Type 1 Diabetes on neonatal microbiomes' by Gajecka et al. in the Diabetologia. Mendeley Supplementary Figure 1. The predicted differently represented pathways in the rectum swabs of women with type 1 diabetes (PICRUSt analysis). The 10 pathways were recognised including 2 pathways from the carbohydrate biosynthesis class. In women with type 1 diabetes decreased folate biosynthesis (N10-formyl-tetrahydrofolate biosynthesis) was noticed. The SCFA production in the rectum swabs of women with type 1 diabetes was found lowered (acetyl-CoA fermentation to butanoate II, purine nucleobases degradation I (anaerobic)). The Generation of Precursor Metabolites and Energy (from superpathway of glycolysis and Entner-Doudoroff) was also decreased. Mendeley Supplementary Table 1. Differentially represented metagenomic pathways, predicted using a PICRUSt bioinformatics software package. Sheet1 presents 165 predicted pathways, significantly differentiated by comparing three vaginal sampling sites of women with T1D with samples of control women. Sheet2 contains 10 pathways predicted as differentially represented in the rectum swabs. Sheet 3 shows the analysis of combined neonatal ear-skin swabs and stool samples. Sheet 4 presents pathways predicted to differ depending on maternal disease status regarding neonatal stool samples. Sheet5 shows pathways of ear-skin swabs differently represented in neonates born to T1D and control mothers. The influence of mode of delivery on pathways prediction is presented in Sheet6. Mendeley Supplementary Table 2. The intake of probiotics during pregnancy in the form of probiotics and/or synbiotics (the qualitative data). The assessment did not take into account the actual amounts of taken probiotic and/or synbiotic supplements, and probiotics and prebiotics from various types of probiotic food products consumed by the surveyed women.本数据集为Gajecka等人发表于《糖尿病学》（Diabetologia）的论文《母体1型糖尿病对新生儿微生物组的影响》的配套数据集。 曼德雷（Mendeley）补充图1：基于PICRUSt分析预测的1型糖尿病女性受试者直肠拭子样本中差异富集的代谢通路。本图共涵盖10条差异通路，其中2条隶属于碳水化合物生物合成类别。在1型糖尿病女性受试者中，观察到叶酸生物合成（N10-甲酰四氢叶酸生物合成）通路表达下调。1型糖尿病女性受试者的直肠拭子样本中，短链脂肪酸（Short-Chain Fatty Acid, SCFA）生成通路活性降低，涉及乙酰辅酶A发酵生成丁酸II通路、嘌呤核苷碱基厌氧降解I通路。前体代谢物与能量生成通路（源自糖酵解与Entner-Doudoroff超通路）的活性同样出现下调。 曼德雷（Mendeley）补充表1：基于PICRUSt生物信息学软件包预测的差异富集宏基因组代谢通路。工作表1收录了165条预测得到的差异通路，该结果通过对比1型糖尿病（Type 1 Diabetes, T1D）女性受试者的3个阴道采样位点样本与健康对照女性样本得出。工作表2包含10条在直肠拭子样本中预测得到的差异通路。工作表3展示了新生儿耳皮肤拭子与粪便样本的联合分析结果。工作表4呈现了针对新生儿粪便样本、随母体疾病状态不同而存在差异的预测通路。工作表5展示了1型糖尿病（T1D）母亲与健康对照母亲所生新生儿的耳皮肤拭子样本中差异富集的代谢通路。工作表6展示了分娩方式对代谢通路预测结果的影响。 曼德雷（Mendeley）补充表2：孕期摄入益生菌及/或合生制剂的相关定性数据。本次评估未统计受试者实际摄入的益生菌/合生制剂补充剂剂量，以及各类益生菌食品中所含的益生菌与益生元摄入量。