Transgene integration typically takes place in an easy-to-transform laboratory variety before the transformation event is introgressed through backcrosses to elite cultivars. As new traits are added t
Study aimed to achieve high levels of multiallelic targeted mutagenesis in sugarcane using CRISPR/Cas9 to target the chlorophyll synthesis gene magnesium chelatase.
Plasmid vector encoding a non-targeting sgRNA sequence under a U6 promoter and a mApple fluorescent reporter. Generated by Vectorbuilder in the pLV[Exp] backbone.