Evidence of DCC activity in somatic cells and pre-meiotic germ cells.

In each cell type we have calculated the median counts for X chromosome genes within 10000 bp of an annotated CES and genes outside 10000 bp, corresponding to Fig 4. In hub, cyst, epithelial, GSC, early and late spermatogonial cells, a two-tailed Wilcoxon test finds that these two sets of genes have statistically different count distributions with an alpha of 0.05. These cell types have a higher ratio of median counts from close and distant genes, indicating that CES sites influence X chromosome gene expression in these cell types, a hallmark of dosage compensation. The cell type with the highest difference between close/distant x gene expression is GSC/early spermatogonia, which also had the highest X/autosome ratio in Table 1. DCC means dosage compensation complex.