Gene resistance to transcriptional reprogramming following nuclear transfer is directly mediated by multiple chromatin repressive pathways. Supplementary Files (Replicate 1/2/4)

Apotome Images (raw data) of 2-cell fertilized mouse embryos with USP21 mRNA injection (inj) or not (T). Embryos are injected at fertilization with mRNA encoding USP21 and subsequently fixed for immunolabelling at the 2-cell stage with anti-H2AK119ub primary antibody (Cell Signaling #8240S + anti-rabbit Cy3-conjugated secondary antibody in red) and DAPI DNA conterstaining (in blue). A total number of 5 replicates were performed on 0140207, 20140211, 20140327, 20140505 and 20140506 (YYYY-MM-DD). In the three first experiments (Replicates 1/2/3), an internal microinjection reporter (HA-tag in green) was also included. In the last two replicates (20140505 and 20140506), co-detection of RING1B protein (Active Motif #39663 + anti-mouse FITC-conjugated secondary antibody in green) was included. Images file format: .zvi本数据集为经USP21信使核糖核酸（mRNA）注射（inj组）或未注射（T组）的2细胞期受精小鼠胚胎的原始Apotome成像图像数据。胚胎于受精时注射编码USP21的mRNA，随后于2细胞期固定样本，采用抗H2AK119ub一抗（Cell Signaling #8240S）结合抗兔Cy3偶联二抗（呈现红色荧光信号）进行免疫标记，并使用4',6-二脒基-2-苯基吲哚（DAPI）进行DNA复染（呈现蓝色荧光信号）。本实验共完成5次生物学重复，分别于2014-02-07、2014-02-11、2014-03-27、2014-05-05及2014-05-06开展。在前三次重复实验（重复1/2/3）中，同步设置了内部微注射报告系统：HA标签（绿色荧光标记）。在后两次重复实验（2014-05-05与2014-05-06）中，额外开展了RING1B蛋白的共检测：使用Active Motif #39663一抗结合抗小鼠异硫氰酸荧光素（FITC）偶联二抗，信号呈绿色。图像文件格式为.zvi