Single-cell RNA sequencing of pancreatic islets from obese mice with different diabetes risk reveals diabetes-susceptible ß cell clusters

Single-cell RNA sequencing of pancreatic islets from 18-week-old male New Zealand Obese (NZO/HIBomDife) and B6.V-Lepob/ob (OB) mice were fed a standard diet or a carbohydrate-enriched diet for 2 additional days (+CH / -CH). Overall design: 4 single-cell RNA sequencing experiments of pancreatic islets of 3 pooled mice per condition were performed using the Chromium Single Cell 3' Reagent Kit v3 (10X Genomics, PN-1000075, Pleasanton, USA), to generate single cell expression profiles of OB and NZO mice with and without a diabetonic treatment.