James R. LaFountain, Rudolf Oldenbourg (2011) CIL:12374, Nephrotoma, Nephrotoma suturalis, primary spermatocyte. CIL. Dataset

Time series images showing the dynamics of kinetchore microtubules during meiosis I in primary spermatocytes of the crane-fly Nephrotoma suturalis that were experimentally flattened. Time-lapse polarization microscopy using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy
(LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 2.0x zoom lens. Images captured every 15 sec using a QImaging Retigo EXi CCD camera. Raw images were processed
using 5-frame algorithm (Shribak and Oldenbourg, 2003). A movie of the time series is included in this grouped set.

时间序列图像展示了蜻蜓科昆虫Nephrotoma suturalis原精细胞中动粒微管在减数分裂I期的动态变化，图像通过实验性压扁获得。采用尼康Microphot SA显微镜，配备液晶偏振光显微镜（LC-PolScope，CRi，马萨诸塞州Woburn）的60x/1.4 PlanApo油浸物镜，1.4 NA油浸聚光镜，以及2.0x放大镜头进行时间延迟偏振显微镜成像。使用QImaging Retigo EXi CCD相机每隔15秒捕捉一张图像。原始图像经过5帧算法（Shribak和Oldenbourg，2003年）处理。本组集包含该时间序列图像的动画。