Fluorescent microscopy images of osteoblasts on () patterned Ti substrates, () complete micron rough Ti substrates, and () complete nano rough Ti substrates after four hours

Osteoblast alignment was detected on the patterned substrates when compared with the entire micron and nano rough Ti substrates. Among the patterned substrates, osteoblast morphology varied. As the nano region width decreased, cells became more round and less diffuse. Magnification = 10×. Nano patterned regions appear darker.<b>Copyright information:</b>Taken from "Nano rough micron patterned titanium for directing osteoblast morphology and adhesion"International Journal of Nanomedicine 2008;3(2):229-241.Published online Jan 2008PMCID:PMC2527665.© 2008 Puckett and Webster, publisher and licensee Dove Medical Press Ltd.