Analgesic effect of the mint terpenoid L-Carvone in sheep

Objective: To determine whether L‑carvone increases the voltage threshold response to a noxious electrical stimulus in sheep. Study Design: Prospective, blinded, randomized, crossover experimental study. Animals: A group of 6 adult sheep. Methods: Sheep were instrumented with cranial dorsothoracic subcutaneous copper electrodes. A stimulator delivered a 10ms square wave at 50 pps starting at 0.1V with a 0.2V/s ramp. The stimulus stopped once two observers who were blinded to treatment noted a behavioral pain response or when a 15V cut-off was reached. Next, 0.15 mL kg−1 of either a 50% L‑carvone solution or a saline-vehicle control was administered intramuscularly, and electrical threshold responses were measured every 5-to-15 min over a 6-hour period using methods identical to the baseline. One week following the first treatment (L‑carvone or control), sheep were studied using identical methods with the second treatment (control or L‑carvone). Drug and time effects were evaluated using..., , , # Analgesic effect of the mint terpenoid L-carvone in sheep **Description of the data and file structure** * Voltage Threshold dryad.xlsx: This file contains the stimulus threshold (in volts) to produce a behavioral response to the noxious stimulus. Responses in animals treated with L-carvone are on the L-carvone data page. Responses for control animals are on the saline data page. - respiratory rate dryad.xlsx: This file contains the respiratory rate (breaths/min) counted before voltage threshold testing at each time point. Responses in animals treated with L-carvone are on the L-carvone data page. Responses for control animals are on the saline data page. * eating periods dryad.xlsx: This file contains the number of periods (out of 28 measurement periods) in which animals were observed eating during treatment with L-carvone and treatment with the saline control. In the data sets, the time indicates the number of minutes following IM (intra-muscular) administration of either L...研究目的：明确L-香芹酮（L-carvone）是否可升高绵羊对伤害性电刺激的电压阈值反应。 研究设计：前瞻性、盲法、随机交叉实验研究。 实验动物：6只成年绵羊。 方法：为绵羊植入颅胸背部皮下铜制电极。采用刺激器以50脉冲每秒（pps）输出10ms方波，初始电压为0.1V，以0.2V/s的速率升压。当两名对实验处理不知情的观察者观察到绵羊出现行为性疼痛反应，或刺激电压达到15V的截止值时，停止电刺激。随后，按0.15mL/kg的剂量肌内（intra-muscular, IM）注射50% L-香芹酮（L-carvone）溶液或生理盐水空白对照。在随后的6小时内，每5至15分钟采用与基线测试完全一致的方法检测电刺激阈值反应。首次处理（L-香芹酮或对照）一周后，采用完全相同的实验流程对绵羊进行第二次处理（对照或L-香芹酮）。采用……方法评估药物与时间的效应。 # 薄荷萜类化合物L-香芹酮（L-carvone）在绵羊中的镇痛作用 数据集与文件结构说明 * Voltage Threshold dryad.xlsx：该文件存储了引发绵羊伤害性电刺激行为反应的刺激阈值（单位：伏特）。L-香芹酮处理组的实验数据存放在该文件的"L-carvone"工作表中，生理盐水对照组的数据存放在"saline"工作表中。 - respiratory rate dryad.xlsx：该文件存储了各时间点电压阈值测试前记录的呼吸频率（单位：次/分钟）。L-香芹酮处理组的实验数据存放在该文件的"L-carvone"工作表中，生理盐水对照组的数据存放在"saline"工作表中。 * eating periods dryad.xlsx：该文件存储了在L-香芹酮处理与生理盐水对照处理期间，观察到动物进食的周期数（共计28个测量周期）。 本数据集中，时间字段代表肌内注射L-香芹酮或生理盐水后经过的分钟数。