Comparison of the lymphocyte before and after the activation.

Immunotherapy using activated lymphocytes has recently been developed, but there is not yet the biomarker which can predict curative effect. In our recent study, we found that random migration is significantly increased in activated lymphocyte. Furthermore, it is suggested that random migration may affect cytotoxicity. Therefore we considered that migration of lymphocyte may become the effect prediction biomarker of Immunotherapy. And then we analyzed the gene which fluctuated by lymphocyte activation to search the molecules which positive regulate migration of lymphocyte. To search the molecules which positively regulate migration of lymphocyte,we prepared a lymphocyte from the human peripheral blood and compared the gene expression before and after the activation.