The core genome m5C methyltransferase JHP1050 (M.Hpy99III) plays an important role in orchestrating gene expression in Helicobacter pylori. The core genome m5C methyltransferase JHP1050 (M.Hpy99III) plays an important role in orchestrating gene expression in Helicobacter pylori

We performed a transcriptome analysis of two Helicobacter pylori wild-type strains and their corresponding mutants lacking a highly conserved GCGC-specific m5C-MTase (JHP1050) that was predicted to be active in all of 459 H. pylori genome sequences analyzed. Transcriptome data revealed that m5C-methylation modifies the transcription of multiple genes related to adherence to host cells, natural competence for DNA uptake or susceptibility to copper.