Evolution of T. cruzi serology and PCR testing in the child contaminated by RBC transfusion.

a) These tests were performed at the Belgian reference center. According to WHO recommendations [19], chronic infection with T. cruzi needs two positive serological assays, using different techniques, to be diagnosed.b) PCR performed as described in Fig 1c) Benznidazole has been given for a duration of two monthsd) Biokit S.A., Barcelona, Spain—Positive result when ratio > 1 (cf. manufacturer instructions)e) ELISA based on soluble extract of T. cruzi trypomastigotes—Positive result if index > 20 (positive control index = 100, negative control index = 0)f) Chagas IFA IgG+IgM, Vircell, Granada, Spain—Positive result when titer ≥ 40 (cf. manufacturer)g) nd: not determinedEvolution of T. cruzi serology and PCR testing in the child contaminated by RBC transfusion.