Rudolf Oldenbourg, James R. LaFountain (2010) CIL:9066, Nephrotoma suturalis, spermatocyte. CIL. Dataset

The events of meiosis I in a living insect spermatocyte beginning at metaphase I with bivalents at the spindle equator followed by anaphase, segregation of half-bivalents diakinesis and commencement of cytokinesis. Time-lapse polarization using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 1.5x zoom lens. Image acquisition and processing Camera: Images captured every 60 sec over 105 min by a QImaging Retigo EXi CCD camera. Raw images were processed using 4-frame algorithm (Shribak and Oldenbourg, 2003). Movie images represent computed retardance as gray values ranging from 0 nm retardance (black) to 2 nm retardance or higher (white).

本研究旨在详细记录活体昆虫精母细胞减数第一次分裂的事件，该过程始于减数第一次中期，双线位于纺锤体赤道，随后进入后期、半双线的分离、次级线期以及胞质分裂的开始。利用配备液晶偏振光显微镜（LC-PolScope，CRi公司，马萨诸塞州沃本）的尼康Microphot SA显微镜，搭载60x/1.4 PlanApo油浸物镜、1.4 NA油浸聚光镜及1.5倍变焦镜头，通过时间分辨极化技术进行成像。图像采集与处理：使用QImaging Retigo EXi CCD相机，以每60秒采集一张图像的方式，持续105分钟，原始图像经过4帧算法（Shribak 和 Oldenbourg，2003年）进行处理。电影图像以灰度值表示计算得到的延迟量，灰度值范围从0 nm延迟（黑色）至2 nm或更高延迟（白色）。