Transcriptomic analysis of APS organoids with and without EGF

EGF is the main mitogen promoting the growth of Kras WT colon cancer organoids. Here we aim to elucidate changes in expression profile of organoids in a suboptimal niche, mimicking the slow proliferation conditions that a tumor senses in vivo APS (APC KO, p53 KO, Smad4 KO) organoids after 7 days of growing in media in absence of EGF. Two different organoids were used, with 2 replicates per organoid. APSMC706 and CTO19 represent two biological replicates of Apc KO p53 KO and Smad4 KO colon cancer organoids. Both of them are derived from C57BL/6 mice, and their mutations were introduced by Crispr.