Forward and reverse primers used to generate pUAST-dRecQ4△ constructs that produce truncated proteins used in Fig. 8#.
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https://figshare.com/articles/dataset/_Forward_and_reverse_primers_used_to_generate_pUAST_dRecQ4_9651_constructs_that_produce_truncated_proteins_used_in_Fig_8_/563952
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#All proteins resulted from above constructs are Flag-tagged.*For constructing pUAST-dRecQ4△868–1207, first PCR using pUAST-dRecQ4△1–1207 as template, with primers listed as in the table, digested with Sal I and Kpn I, then ligated into pUAST-dRecQ4△868–1579 vector which had been digested with Xho I and Kpn I.
创建时间：
2015-12-02
