Substrate-specific induction of selected carbohydrase genes.

1aga16B, Sde_1175; algF, Sde_2873; cel5H, Sde_3237; chi18A, Sde_1704; xyn11A, Sde_0701
2S. degradans 2-40 was grown in minimal medium supplemented by 0.2% glucose to an OD600 of 0.33–0.35. The cells were harvested and transferred into fresh medium containing 0.2% xylan, chitin, alginic acid, Avicel, or glucose or 0.1% agar as indicated. After 4 hr, total RNA was extracted, converted to a cDNA copy and transcript levels relative to initial levels estimated by qRT-PCR using gene-specific primer pairs as described in the Materials and Methods. Transcript levels were normalized to guanylate kinase (Sde_3695) transcript levels.