Determining sensitivity of the Target Selector Assay at or near single copy input levels.

Column ‘ddPCR Copies (Reference)’ shows ddPCR validation of controls for cell lines containing mutations of interest. Source DNA was isolated from named cell lines or commercially sourced human placental DNA. Copy numbers per reaction for Standards A-D were determined by independent measurements post dilution using droplet digital PCR. Target Selector results at stochastic sampling levels show qPCR copies as determined by the maximum likelihood estimate of the standard curve, the number of empty well and replicates run per concentration, the percentage of empty wells and the copy number as calculated using the Poisson statistic.