Suppression of CHIKV and DENV replication is evident in clonal cell populations expressing antiviral Dual Virus Targeting Group I Intron Constructs.

Clonal Ae. albopictus C6/36 cells, transformed with the single or dual targeting antiviral intron constructs indicated, were challenged with each arbovirus indicated. Infected cell supernatants were collected, and viral titers were determined by TCID50-IFA. I = wild type C6/36 mosquito cells infected with virus indicated; U = uninfected wild type C6/36 mosquito cells; ΔC/D = ΔCHIKV/DENVv1; ΔD/C = ΔDENV/CHIKVv1. Supernatants originating from each clonal cell population were used as inoculum for three wells of fresh C6/36 cells and analyzed for the presence of each DENV serotype of CHIKV vaccine strain 181/25 by TCID50-IFA. The experiment was performed at three independent times resulting in a total of nine replicates for each dual-targeting intron-ΔN Bax tested.