Procyanidin B1 Conducted Transcriptional Reprogramming and Anti-inflammatory Response in Chicken HD11 Cells Through Transcription Factor STAT2 and HIF1A

We used LPS-stimulated chicken HD11 macrophage-like cell as a model to identify the key transcription factors involved in transcriptome regulation responsible for Procyanidin B1 treatment. RNA-seq identified 1,384 transcripts significantly differentially expressed between the Procyanidin B1 treated group and the control group, and 696 transcripts significantly differentially expressed between LPS+Procyanidin B1 and LPS treated group (FDR < 0.05, FDR > 1.5). Bioinformatic analysis revealed that five transcription factors, FOSL1::JUND, CREB1, ZNF467, STAT2, and HIF1A were candidate genes for transcriptome regulation in Procyanidin B1 treated HD11 cells. Overall design: The samples for RNA-seq experiment were collected from four treatment groups with four replicates for each group, the Procyanidin B1, Procyanidin B1+LPS, LPS and Control.